HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV6	E1^E4	CCTTGTGTCCTCAAGCACCA	AGG	F	77	96	55	62.24
2	HPV6	E1^E4	GGTGCTTGAGGACACAAGGG	TGG	R	182	201	60	54.31
3	HPV6	E1^E4	CTTGGTGCTTGAGGACACAA	GGG	R	179	198	50	44.35
4	HPV6	E1^E4	GTATGTAGTAGATTCAGGAA	TGG	R	221	240	35	44.24
5	HPV6	E1^E4	GCTTGAGGACACAAGGGTGG	AGG	R	185	204	60	41.11
6	HPV11	E1^E4	GGTGCAGGCGGACACTGTAG	GGG	R	176	195	65	62.19
7	HPV11	E1^E4	GTATGTAGTAGGTTCAGCAA	TGG	R	218	237	40	47.95
8	HPV11	E1^E4	TACAGTGTCCGCCTGCACCA	CGG	F	80	99	60	46.98
9	HPV11	E1^E4	TGTGGCCAACATCAGATCCG	TGG	F	167	186	55	40.41
10	HPV40	E1^E4	GGGGGCGTCGGGGGTGTCGG	TGG	R	203	222	85	55.62
11	HPV40	E1^E4	GGTGTCGGTGGCAGTGGTCG	GGG	R	215	234	70	51.97
12	HPV40	E1^E4	GGCGTCAGCAGTCTCAACAA	TGG	R	242	261	55	51.54
13	HPV40	E1^E4	GGCGGGGCCGACCGTTTCGG	TGG	R	170	189	80	49.52
14	HPV40	E1^E4	CCTGACCCTGAAAGCAACAA	CGG	F	243	262	50	47.63
15	HPV40	E1^E4	ACAGCGACCACCGAAACGGT	CGG	F	105	124	60	44.97
16	HPV40	E1^E4	ACTGTGGGCAAATCACTCGG	AGG	F	186	205	55	44.53
17	HPV40	E1^E4	GGGGTGTCGGTGGCAGTGGT	CGG	R	213	232	70	43.71
18	HPV40	E1^E4	GGGCACAGGAACTGTGGTGG	AGG	F	264	283	65	42.93
19	HPV42	E1^E4	GCGTAGCCTGACTTCCACAG	TGG	R	7	26	60	57.86
20	HPV43	E1^E4	GGTGCACGTGGAATCTGGGA	CGG	R	194	213	60	58
21	HPV43	E1^E4	GGTTGTGCCCTCTAGGGTGG	TGG	R	25	44	65	54.21
22	HPV43	E1^E4	CACCCTAGAGGGCACAACCG	TGG	F	249	268	65	50
23	HPV43	E1^E4	AGATTCAGTAGACGCAACAG	AGG	R	236	255	45	43.05
24	HPV43	E1^E4	GCGTCTACTGAATCTGACAC	CGG	F	42	61	50	42.67
25	HPV44	E1^E4	CCGCCATGTAAACGACCCCG	AGG	F	138	157	65	62.31
26	HPV44	E1^E4	GGTGGTGGTGGTGGAGGAGG	AGG	R	227	246	70	61.72
27	HPV44	E1^E4	GGATGAGGCCTGTGCAAGGG	TGG	R	209	228	65	58.85
28	HPV44	E1^E4	ACTGCCCATTGGCACCGCCG	AGG	F	101	120	70	51.37
29	HPV44	E1^E4	TGTCAGAAACAGCGACTCCG	TGG	F	200	219	55	47.81
30	HPV44	E1^E4	GCCTCATCCACACTGCCCAT	TGG	F	90	109	60	47.12
31	HPV44	E1^E4	GGCGTTGTTGGTGTTTGTGG	GGG	R	122	141	55	45.08
32	HPV44	E1^E4	GGCCTGTGCAAGGGTGGTGG	TGG	R	215	234	70	43.56
33	HPV44	E1^E4	TGGCACCGCCGAGGACTGCG	TGG	F	110	129	75	41.36
34	HPV54	E1^E4	GGAGTTGTCGGTACGTGGTG	TGG	R	176	195	60	66.64
35	HPV54	E1^E4	ACACAGCACCTCAGACCCCG	TGG	F	170	189	65	60.12
36	HPV54	E1^E4	CTAGCTCGTTTTCTAGGCGG	CGG	R	132	151	55	48.47
37	HPV61	E1^E4	GGGTGGACAGTACTGACCAG	TGG	F	220	239	60	70.14
38	HPV61	E1^E4	GGGGGTGGTGGTGGTCTGTG	TGG	R	206	225	70	58.23
39	HPV61	E1^E4	GGGTGGGGCCCACGCACGTG	GGG	R	187	206	80	53.11
40	HPV61	E1^E4	GGTGGGGCCCACGCACGTGG	GGG	R	188	207	80	51.46
41	HPV61	E1^E4	GGGGCCCACGCACGTGGGGG	TGG	R	191	210	85	47.19
42	HPV70	E1^E4	GTAAAGGCTGCAACAAACGA	CGG	F	193	212	45	49.91
43	HPV70	E1^E4	GGTGCATGGGGTTGTTGCGG	CGG	R	140	159	65	41.48
44	HPV70	E1^E4	TGTTGCGGCGGTATGGGCCG	GGG	R	152	171	70	40.12
45	HPV72	E1^E4	GGCGTGACAGTGCTGACCAG	TGG	F	223	242	65	56.48
46	HPV72	E1^E4	GGGGCCCACGCACGTGGCGG	TGG	R	200	219	85	47.26
47	HPV81	E1^E4	GGGCCCCGCCAAGACCACCA	AGG	F	107	126	75	62.19
48	HPV81	E1^E4	GGTGGACAATAACAAATCAG	GGG	F	215	234	40	62.05
49	HPV81	E1^E4	GGTACTGGTGGCGGGTTCGG	GGG	R	203	222	70	53.39
50	HPV81	E1^E4	GGGGCCTTGGTGGTCTTGGC	GGG	R	168	187	70	50.02
51	HPV81	E1^E4	GGGCCTTGGTGGTCTTGGCG	GGG	R	169	188	70	49.45
52	HPV81	E1^E4	GGGGCCCACGTGCTGGGTAC	TGG	R	188	207	75	45.48
53	HPV81	E1^E4	TGCACCACCGAACATCTGGG	TGG	F	197	216	60	44.83
54	HPV81	E1^E4	GGGTGGACAATAACAAATCA	GGG	F	214	233	40	40.85